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Overview: Tris brand name is tris(hydroxymethyl)aminomethane; tromethamine; tromethamine; 2-amino-2-(hydroxymethyl)-1,3-propanediol. It is a white crystal or powder. Soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.

Indications: Tromethamine is a sodium-free amino buffer base, which reacts with H2CO3 in body fluids to reduce H2CO3 and generate HCO32- at the same time. It can absorb hydrogen ions and correct acidemia. Strong, and can penetrate the cell membrane, commonly used in acute metabolic and respiratory acidemia.

Buffering properties: Tris is a weak base with a pKa of 8.1 at 25°C; according to buffer theory, the effective buffering range of Tris buffer is between pH 7.0 and 9.2. The pH of the aqueous solution of Tris base is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value, and then the buffer solution with the pH value can be obtained. However, attention should be paid to the effect of temperature on the pKa of Tris.

Application: Tris is widely used in acute metabolic and respiratory acidemia. It is an alkaline buffer and has a good buffering effect on metabolic acidosis and enzymatic activity. Tris is often used as a biological buffer and is often formulated with pH values ​​of 6.8, 7.4, 8.0, and 8.8. Its structural formula and pH value vary greatly with temperature. In general Chemicalbook says that for every degree increase in temperature, the pH decreases by 0.03. Tris is widely used in the preparation of buffers in biochemical and molecular biology experiments. For example, Tris is required in both TAE and TBE buffers (for solubilization of nucleic acids) commonly used in biochemical experiments. Since it contains an amino group, it can undergo condensation reactions with aldehydes.