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Thrombin CAS:9002-04-4

Short Description:

Catalog Number: XD90400
CAS: 9002-04-4
Molecular Formula: C6H14O6
Molecular Weight: 182.17
Availability: In Stock
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Prepack: 5g USD10
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Product Detail

Product Tags

Catalog Number XD90400
Product Name Thrombin
CAS 9002-04-4
Molecular Formula C6H14O6
Molecular Weight 182.17
Storage Details 2 to 8 °C
Harmonized Tariff Code 30021900

 

Product Specification

Melting Point 166-168 deg C
Appearance White powder

 

PH sensors were fabricated by anodically electrodepositing iridium oxide films (AEIROFs) onto microelectrodes on chips and coated with poly(ethyleneimine) (PEI) for mechanical stability. These demonstrate super-Nernstian response to pH from pH 4.0 to 7.7 in chloride-free phosphate buffer. The surface of the chip was coated with fibronectin for the attachment of porcine aortic endothelial cells (PAECs). The working capability of the pH sensor for monitoring acute local pH changes was investigated by stimulating the PAECs with thrombin. Our results show that thrombin induced acute extracellular acidification of PAECs and dissolution of fibronectin, causing the local pH to decrease. The use of PD98059, a mitogen-activated protein kinase (MAPK) inhibitor, reduced extracellular acidification and an increase in local pH was observed. This study shows that our pH sensors can facilitate the investigation of acute cellular responses to stimulation by monitoring the real-time, local pH changes o f cells attached to the sensors.

 


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    Thrombin CAS:9002-04-4