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SYBR GREEN I Nucleic Acid Gel Stain Cas: 163795-75-3 99% Solid

Short Description:

Catalog Number: XD90236
Cas: 163795-75-3
Molecular Formula: C32H37N4S
Molecular Weight: 509.728
Availability: In Stock
Price:  
Prepack: 5g USD10
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Product Detail

Product Tags

Catalog Number XD90236
Product Name SYBR GREEN I Nucleic Acid Gel Stain

CAS

163795-75-3

Molecular Formula

C32H37N4S

Molecular Weight

509.728
Storage Details -20°C

 

Product Specification

Appearance White powder
Assay 99%

 

Flow cytometry is an objective method for conducting in vitro antimalarial sensitivity assays with increasing potential for application in field sites. We examined in vitro susceptibility to seven anti-malarial drugs for 40 fresh P. falciparum field isolates via a flow cytometry method (FCM), a colorimetric LDH-based ELISA : DELI), and standard microscopic slide analysis of growth. For FCM, 184/280 (66%) assays met analytical acceptance criteria, compared to 166/280 (59%) for DELI. There was good agreement between FCM and microscopy, while DELI tended to produce higher half-maximal inhibition constants (IC50s) than FCM, with an overall bias of 2.2-fold (Bland-Altman comparison). Values for artesunate and dihydroartemisinin were most affected. Paradoxical increases in signal at very high concentrations of mefloquine and related compounds were more marked with the DELI assay, suggesting that off-target effects on LDH production may be responsible. Loss of FCM signal due to reinvasion or slow growth was observed in a small number of samples. These results extend previous work on use of flow cytometry to determine antimalarial susceptibility in terms of the number of samples, range of drugs, and comparison with other methods.


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    SYBR GREEN I Nucleic Acid Gel Stain Cas: 163795-75-3 99% Solid