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Polymyxin B sulfate CAS:1405-20-5 White powder

Short Description:

Catalog Number: XD90360
CAS: 1405-20-5
Molecular Formula: C55H96N16O13 · 2H2SO4
Molecular Weight: 1385.61
Availability: In Stock
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Prepack: 5g USD20
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Product Detail

Product Tags

Catalog Number XD90360
Product Name Polymyxin B sulfate
CAS 1405-20-5
Molecular Formula C55H96N16O13 · 2H2SO4
Molecular Weight 1385.61
Storage Details 2 to 8 °C
Harmonized Tariff Code 29419000

 

Product Specification

Conclusion Conforms to USP35
Heavy metals <20ppm
pH 5-7
Identification Liquid chromatography: conforms
Assay 99%
Appearance White powder
Loss on Drying <6%
Solubility Freely soluble in water, slightly soluble in Ethanol
Sulfate 15.5% - 17.5%
Particle Size <30µm
Specific optical rotation -78° -90°
Phenylalanine 9.0%-12.0%
Pyrogens Meets the requirements
Sulfated ash <0.75%
Total viable aerobic count <100cfu/g
Potency >6500 IU/mg

 

The ExPortal protein secretion organelle in Streptococcus pyogenes is an anionic phospholipid-containing membrane microdomain enriched in Sec translocons and postsecretion protein biogenesis factors. Polymyxin B binds to and disrupts ExPortal integrity, resulting in defective secretion of several toxins. To gain insight into factors that influence ExPortal organization, a genetic screen was conducted to select for spontaneous polymyxin B-resistant mutants displaying enhanced ExPortal integrity. Whole-genome resequencing of 25 resistant mutants revealed from one to four mutations per mutant genome clustered primarily within a core set of 10 gene groups. Construction of mutants with individual deletions or insertions demonstrated that 7 core genes confer resistance and enhanced ExPortal integrity through loss of function, while 3 were likely due to gain of function and/or combinatorial effects. Core resistance genes include a transcriptional regulator of lipid biosynthesis, several genes involved in nutrient acquisition, and a variety of genes involved in stress responses. Two members of the latter class also function as novel regulators of the secreted SpeB cysteine protease. Analysis of the most frequently isolated mutation, a single nucleotide deletion in a track of 9 consecutive adenine residues in pstS, encoding a component of a high-affinity Pi transporter, suggests that this sequence functions as a molecular switch to facilitate stress adaptation. Together, these data suggest the existence of a membrane stress response that promotes enhanced ExPortal integrity and resistance to cationic antimicrobial peptides.

 


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    Polymyxin B sulfate CAS:1405-20-5 White powder