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Penicillin G potassium salt CAS:113-98-4 99% White powder

Short Description:

Catalog Number: XD90351
Cas: 113-98-4
Molecular Formula: C16H17KN2O4S
Molecular Weight: 372.48
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Product Detail

Product Tags

Catalog Number XD90351
Product Name Penicillin G potassium salt
CAS 113-98-4
Molecular Formula C16H17KN2O4S
Molecular Weight 372.48
Storage Details Ambient
Harmonized Tariff Code 29411000

 

Product Specification

Loss on Drying <1.0%
Colour of Solution Not more intensely coloured than intensity Y2 or YG2 of the range of reference
pH 5-7.5
Related Substances <1.0%
Assay 99%
Potency 1440 - 1680u/mg
Appearance White to almost white crystalline powder
Clarity of Solution Not more than opalescent than reference suspension 1
Transmittance NLT 90%
Butyl acetate NMT 0.05%
Butanol NMT 0.12%

e described previously that an autoinducer molecule, identified as 1,3-diaminopropane (1,3-DAP), is secreted by Penicillium chrysogenum and Acremonium chrysogenum. Using pH-controlled fermentor cultures we have observed in this work that 1,3-DAP and spermidine clearly stimulate the biosynthesis of benzylpenicillin in P. chrysogenum, both in defined and in complex penicillin production media. Both 1,3-DAP and spermidine, but not putrescine (1,4-diaminobutane), produce a drastic increase in the transcript levels of the penicillin biosynthetic genes pcbAB, pcbC and penDE. These polyamines do not affect the expression of the global pH-stress regulator pacC gene, thus excluding that the effect of 1,3-DAP and spermidine is due to a modification of the pH control mechanism. Expression of the three penicillin biosynthetic genes is drastically reduced in a laeA-knock-down mutant of P. chrysogenum, which produces very low levels of benzylpenicillin. Interestingly, 1,3-DAP and spermidine revert t he effect of the laeA knock-down mutation, completely restoring the levels of penicillin production. Furthermore, 1,3-DAP and spermidine enhanced the expression of laeA in the parental strain and restored the levels of laeA transcripts in the laeA knock-down mutant. Taken together these results indicate that the stimulatory effect of the inducer molecules 1,3-DAP and spermidine is exerted, at least in part, through the stimulation of the expression of laeA, a global regulator that acts epigenetically on the expression of secondary metabolite genes by heterochromatin reorganization.


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    Penicillin G potassium salt CAS:113-98-4 99% White powder