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oomassie brilliant blue R-250 Cas: 6104-59-2 Blue to dark blue powder

Short Description:

Catalog Number: XD90541
Cas: 6104-59-2
Molecular Formula: C45H44N3NaO7S2
Molecular Weight: 825.97
Availability: In Stock
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Prepack: 25g USD10
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Product Detail

Product Tags

Catalog Number XD90541
Product Name oomassie brilliant blue R-250

CAS

6104-59-2

Molecular Formula

C45H44N3NaO7S2

Molecular Weight

825.97
Storage Details Ambient
Harmonized Tariff Code 32129000

Product Specification

Appearance

Blue to dark blue powder

 

We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E(550-535)) of 19.7+/-6.3 mM(-1) cm(-1) and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 +/- 122 micromol min(-1) mg(-1) protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus.2009 Elsevier Inc. All rights reserved.


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    oomassie brilliant blue R-250 Cas: 6104-59-2 Blue to dark blue powder