oomassie brilliant blue R-250 Cas: 6104-59-2 Blue to dark blue powder
Catalog Number | XD90541 |
Product Name | oomassie brilliant blue R-250 |
CAS |
6104-59-2 |
Molecular Formula |
C45H44N3NaO7S2 |
Molecular Weight |
825.97 |
Storage Details | Ambient |
Harmonized Tariff Code | 32129000 |
Product Specification
Appearance |
Blue to dark blue powder |
We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E(550-535)) of 19.7+/-6.3 mM(-1) cm(-1) and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 +/- 122 micromol min(-1) mg(-1) protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus.2009 Elsevier Inc. All rights reserved.