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Online Exporter Ados - Guanidine Hydrochloride Cas: 50-01-1 99% White powder – XD BIOCHEM

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We have state-of-the-art equipment. Our products are exported for the USA, the UK and so on, enjoying a fantastic status among the clients for D-(+)-Cellobiose, D-Fucose, D-Fucose, Aggressive price with top quality and satisfying support make us earned extra customers.we wish to work along with you and request common enhancement.
Online Exporter Ados - Guanidine Hydrochloride Cas: 50-01-1 99% White powder – XD BIOCHEM Detail:

Catalog Number XD90198
Product Name Guanidine Hydrochloride

CAS

50-01-1

Molecular Formula

CH6N3Cl

Molecular Weight

95.5388
Storage Details Ambient
Harmonized Tariff Code 29252900

 

Product Specification

Melting Point 185.3 – 187.5 Deg C
Water <0.3%
Solubility Clear colourless solution at 6M in Water
Assay >99%
UV Absorbance 0.10 at 260nm
Appearance White powder
IR spectrum Conforms

 

One of the factors preventing clinical application of regenerative medicine to degenerative cartilage diseases is a suitable source of cells. Chondrocytes, the only cell type of cartilage, grown in vitro under culture conditions to expand cell numbers lose their phenotype along with the ability to generate hyaline cartilaginous tissue. In this study we determine that a serum- and growth-factor-free three-dimensional (3D) culture system restores the ability of the passaged chondrocytes to form cartilage tissue in vitro, a process that involves sox9.Bovine articular chondrocytes were passaged twice to allow for cell number expansion (P2) and cultured at high density on 3D collagen-type-II-coated membranes in high glucose content media supplemented with insulin and dexamethasone (SF3D). The cells were characterized after monolayer expansion and following 3D culture by flow cytometry, gene expression, and histology. The early changes in signaling transduction pathways during redifferentiat ion were characterized.The P2 cells showed a progenitor-like antigen profile of 99% CD44(+) and 40% CD105(+) and a gene expression profile suggestive of interzone cells. P2 in SF3D expressed chondrogenic genes and accumulated extracellular matrix. Downregulating insulin receptor (IR) with HNMPA-(AM3) or the PI-3/AKT kinase pathway (activated by insulin treatment) with Wortmannin inhibited collagen synthesis. HNMPA-(AM3) reduced expression of Col2, Col11, and IR genes as well as Sox6 and -9. Co-immunoprecipitation and chromatin immunoprecipitation analyses of HNMPA-(AM3)-treated cells showed binding of the coactivators Sox6 and Med12 with Sox9 but reduced Sox9-Col2a1 binding.We describe a novel culture method that allows for increase in the number of chondrocytes and promotes hyaline-like cartilage tissue formation in part by insulin-mediated Sox9-Col2a1 binding. The suitability of the tissue generated via this approach for use in joint repair needs to be examined in vivo.

 


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    Online Exporter Ados - Guanidine Hydrochloride Cas: 50-01-1 99% White powder – XD BIOCHEM