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One of Hottest for Hdaos - Guanidine Thiocyanate Sulfate Cas: 593-84-0 99% White to off-white crystalline powder – XD BIOCHEM

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One of Hottest for Hdaos - Guanidine Thiocyanate Sulfate Cas: 593-84-0 99% White to off-white crystalline powder – XD BIOCHEM Detail:

Catalog Number XD90199
Product Name Guanidine Thiocyanate Sulfate 

CAS

593-84-0

Molecular Formula

C2H4N4S

Molecular Weight

116.145
Storage Details Ambient
Harmonized Tariff Code 29299000

 

Product Specification

Melting Point 115 – 121 Deg C
Assay >99%
Cl <1%
Appearance White to off-white crystalline powder
Water KF <0.5%

 

Mycobacterium tuberculosis can persist for decades in the human host. Stringent response pathways involving inorganic polyphosphate [poly(P)], which is synthesized and hydrolyzed by polyphosphate kinase (PPK) and exopolyphosphatase (PPX), respectively, are believed to play a key regulatory role in bacterial persistence. We show here that M. tuberculosis poly(P) accumulation is temporally linked to bacillary growth restriction. We also identify M. tuberculosis Rv1026 as a novel exopolyphosphatase with hydrolytic activity against long-chain poly(P). Using a tetracycline-inducible expression system to knock down expression of Rv1026 (ppx2), we found that M. tuberculosis poly(P) accumulation leads to slowed growth and reduced susceptibility to isoniazid, increased resistance to heat and acid pH, and enhanced intracellular survival during macrophage infection. By transmission electron microscopy, the ppx2 knockdown strain exhibited increased cell wall thickness, which was associated with re duced cell wall permeability to hydrophilic drugs rather than induction of drug efflux pumps or altered biofilm formation relative to the empty vector control. Transcriptomic and metabolomic analysis revealed a metabolic downshift of the ppx2 knockdown characterized by reduced transcription and translation and a downshift of glycerol-3-phosphate levels. In summary, poly(P) plays an important role in M. tuberculosis growth restriction and metabolic downshift and contributes to antibiotic tolerance through altered cell wall permeability.The stringent response, involving the regulatory molecules inorganic polyphosphate [poly(P)] and (p)ppGpp, is believed to mediate Mycobacterium tuberculosis persistence. In this study, we identified a novel enzyme (Rv1026, PPX2) responsible for hydrolyzing long-chain poly(P). A genetically engineered M. tuberculosis strain deficient in the ppx2 gene showed increased poly(P) levels, which were associated with early bacterial growth arrest and reduced susce ptibility to the first-line drug isoniazid, as well as increased bacterial survival during exposure to stress conditions and within macrophages. Relative to the control strain, the mutant showed increased thickness of the cell wall and reduced drug permeability. Global gene expression and metabolite analysis revealed reduced expression of the transcriptional and translational machinery and a shift in carbon source utilization. In summary, regulation of the poly(P) balance is critical for persister formation in M. tuberculosis.


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Our crew through skilled training. Skilled skilled knowledge, strong sense of company, to meet the company wants of customers for One of Hottest for Hdaos - Guanidine Thiocyanate Sulfate Cas: 593-84-0 99% White to off-white crystalline powder – XD BIOCHEM , The product will supply to all over the world, such as: Angola, Milan, Benin, We put the product quality and customer's benefits to the first place. Our experienced salesmen supply prompt and efficient service. Quality control group make sure the best quality. We believe quality comes from detail. If you have demand, let us work together to get success.
  • The accounts manager made a detailed introduction about the product, so that we have a comprehensive understanding of the product, and ultimately we decided to cooperate.
    5 Stars By Teresa from Burundi - 2018.06.09 12:42
    This supplier's raw material quality is stable and reliable, has always been in accordance with the requirements of our company to provide the goods that quality meet our requirements.
    5 Stars By Elva from Romania - 2017.11.01 17:04
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    One of Hottest for Hdaos - Guanidine Thiocyanate Sulfate Cas: 593-84-0 99% White to off-white crystalline powder – XD BIOCHEM