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Chlorophenol Red-Beta-D-Galactopyranoside, sodium salt is a synthetic compound commonly used in biochemical and molecular biology applications. It serves as a chromogenic substrate for the enzyme β-galactosidase, enabling researchers to study enzyme activity in various biological samples.

Chemical Structure and Properties

This compound features a chlorophenol group conjugated with a beta-D-galactopyranoside unit. The presence of the chlorophenol moiety is crucial as it allows for colorimetric detection; upon hydrolysis by β-galactosidase, the compound releases chlorophenol, which produces a distinct color change. The sodium salt form enhances solubility in aqueous solutions, making it suitable for experimental use.

Applications

Chlorophenol Red-Beta-D-Galactopyranoside is extensively utilized in laboratory settings for several purposes:

Enzyme Activity Assays: It serves as an indicator for measuring β-galactosidase activity, a significant enzyme in lactose metabolism.

Molecular Cloning: In cloning experiments, this substrate is often employed in blue/white screening processes. Bacterial colonies expressing the lacZ gene will convert the substrate, leading to a color change that indicates successful transformation.

Research Studies: Researchers use this compound in studies related to genetic expression, metabolic pathways, and cellular functions involving glycosidases.

Conclusion

Chlorophenol Red-Beta-D-Galactopyranoside, sodium salt is a versatile and valuable tool in the biochemical field. Its ability to facilitate colorimetric assays makes it an essential compound for enzymatic studies and genetic engineering applications. As research continues to evolve, compounds like this play a critical role in advancing our understanding of biological processes.