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Introduction: BSA is one of the most commonly used proteins in biochemical laboratories, and its importance may be overlooked in experiments because it is too common and too mundane. Bovine serum albumin (BSA), also known as the fifth component, is a globulin in bovine serum containing 583 amino acid residues, with a molecular weight of 66.430kDa and an isoelectric point of 4.7. BSA has a wide range of applications in biochemical experiments, such as as a Blocking agent in western blots.

Application: Bovine Serum Albumin (BSA), also known as the fifth component, is a globulin in bovine serum, containing 607 amino acid residues, and has a wide range of applications in biochemical experiments. Bovine serum albumin is generally used as a stabilizer in the storage solution and reaction solution of restriction enzymes or modified enzymes to stabilize enzyme activity and prevent enzyme decomposition and non-specific adsorption.

Function: BSA is generally used as a stabilizer in the storage solution and reaction solution of restriction enzymes or modified enzymes, because some enzymes are unstable or have low activity at low concentrations. After adding BSA, it may play the role of “protection” or “carrier”, and the activity of many enzymes can be greatly improved after adding BSA. Enzymes that do not require the addition of BSA are generally not affected by the addition of BSA. For most substrate DNA, BSCA can make the digestion more complete, and can achieve repeated cutting. At 37°C, when the digestion reaction exceeds 1 h, BSA can make the enzyme more stable, because in the reaction buffer without BSA, many restriction enzymes can only survive at 37°C for 10~20min or even shorter time. . In contrast, BSA can bind metal ions and other chemicals in buffer or substrate DNA that inhibit the activity of restriction endonucleases.

Uses: Standard grade bovine serum albumin (BSA, StandardGrade), which can meet the needs of most routine experiments, such as immunoblocking agent, tissue cell (microbial animal and insect cells, etc.) culture nutrients and culture components, protein/enzyme stabilization reagents and protein quantification standards. Diagnostic grade bovine serum albumin (BSA, DiagnosticGrade) can meet most routine experimental needs, such as immunoblocking agent, protein/enzyme stabilizer, diluent, carrier and protein standard. In addition, it can also be used for immunoassays, cell culture and hybridization experiments that require high sensitivity.