Products

N-alpha-FMOC-L-glutamic acid alpha-t-butyl ester, also known as Fmoc-Glu(OtBu)-OH, is a vital compound used in peptide synthesis. It serves as a building block for the assembly of peptides and protects the glutamic acid residue during the synthesis process.One of the primary effects of Fmoc-Glu(OtBu)-OH is its ability to safeguard the carboxylic acid group of glutamic acid. The t-butyl (OtBu) ester group acts as a protecting group, preventing undesired reactions with other reagents during peptide coupling reactions. This selective protection ensures that the desired peptide sequence is obtained and allows for controlled peptide assembly.Fmoc-Glu(OtBu)-OH is especially valuable in solid-phase peptide synthesis (SPPS). In SPPS, protected amino acids are stepwise added to a solid support, such as a resin, to build the peptide chain. Fmoc-Glu(OtBu)-OH can be efficiently coupled with other amino acids using standard peptide synthesis protocols. Its stability during the coupling reactions ensures the proper incorporation of the glutamic acid residue into the peptide sequence.Furthermore, Fmoc-Glu(OtBu)-OH contributes to the overall stability and efficiency of the peptide synthesis process. The OtBu protecting group prevents side reactions, such as racemization or undesired coupling reactions, from occurring during peptide assembly. This protection improves the purity and yield of the synthesized peptide.When the desired peptide sequence has been synthesized, the Fmoc and protecting groups, including the OtBu group on the glutamic acid residue, can be selectively removed using appropriate deprotection reagents. This allows for the recovery of the free carboxylic acid group of glutamic acid, which can be further modified or utilized for downstream applications.Fmoc-Glu(OtBu)-OH finds applications in various fields, including peptide-based drug discovery, chemical biology, and biochemistry. It enables the synthesis of peptides with glutamic acid residues, which are crucial for numerous biological processes. By incorporating Fmoc-Glu(OtBu)-OH into peptide sequences, researchers can investigate the structure-function relationships of proteins and peptides or develop peptide-based drugs with specific biological activities.In summary, N-alpha-FMOC-L-glutamic acid alpha-t-butyl ester (Fmoc-Glu(OtBu)-OH) is a critical compound used in peptide synthesis. Its ability to protect the glutamic acid residue, compatibility with SPPS, and stability during synthesis make it a valuable building block in the production of peptides containing glutamic acid. Fmoc-Glu(OtBu)-OH enables precise control over peptide sequences and facilitates the synthesis of bioactive peptides for various research and pharmaceutical applications.