N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly Cas:64967-39-1 99% White to off-white powder
Catalog Number | XD90187 |
Product Name | N-[3-(2-Furyl)acryloyl]-Phe-Gly-Gly |
CAS | 64967-39-1 |
Molecular Formula | C20H21N3O6 |
Molecular Weight | 399.39 |
Storage Details | -15 to -20 °C |
Harmonized Tariff Code | 29321900 |
Product Specification
Appearance | White to off-white powder |
Assay | ≥99% |
Melting point | 245 °C (dec.)(lit.) |
Solubility | NH4OH 1 M: 50 mg/mL, clear to slightly turbid, yellow
PSA 137.74000 |
In the kinetic angiotensin-converting enzyme (ACE) method, a practical and optimal buffer is 80 mmol/L borate buffer at pH 8.2 (37 degrees C). A lag phase is detected in the reaction, and a 5-min incubation of substrate and plasma is suggested before the kinetic measurement. The substrate, N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), concentration is maximized at 1.0 mmol/L and the measurement wavelength is at 345 nm to ensure linearity of measurement. The proposed procedure uses a 1:9 plasma-to-reagent volume ratio. The linear range of the assay extends to approximately 170 U/L, representing a 25% substrate hydrolysis. The FAPGG absorptivity is determined by measuring the difference in absorbance between 1.0 mmol/L FAPGG and the product solutions. The wavelength fidelity is checked by noting the expected absorbance value of the FAPGG solution, and a 1.0-nm deviation from 345 nm alters the absorbance by 15.5%. The precision of ACE assays at approximately 60 and 100 U/L is 3.5% and 2.4% within batch and 2.9% and 2.6% between batch, respectively. The reference interval (2.5th to 97.5th percentiles) is 41-139 U/L, and there is no difference between values for men and women.