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Lipase from porcine pancreas CAS:9001-62-1 Brown to beige powder

Short Description:

Catalog Number: XD90387
CAS: 9001-62-1
Molecular Formula: N/A
Molecular Weight: N/A
Availability: In Stock
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Prepack: 100g USD10
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Product Detail

Product Tags

Catalog Number XD90387
Product Name Lipase from porcine pancreas
CAS 9001-62-1
Molecular Formula N/A
Molecular Weight N/A
Storage Details 2 to 8 °C
Harmonized Tariff Code 35079090

 

Product Specification

Ash <12%
AS <2mg/kg
Pb <2mg/kg
Water Content <8%
Assay 99%
Enzyme Activity >30000u/g
Appearance Brown to beige powder
Coliform Group <30MPH/100g
For research use only, not for human use research use only, not for human use

 

 

The alteration of triglyceride (TG) metabolism in vascular smooth muscle cells (SMC) is likely to be correlated with certain phenotype, though this has not been elucidated. Adipose triglyceride lipase (ATGL) exerts major TG catalytic activity in both adipotic and non-adipotic cells. In the present study, we isolated SMC from ATGL-deficient mice (ATGL(-/-)mSMC). ATGL(-/-)mSMC showed spontaneous TG accumulation with lower mitogenic response and smooth muscle actin (SMA) expression compared to ATGL (+/+)mSMC. Percentage of senescence-associated _-galactosidase positive cells was also increased in ATGL(-/-)mSMC. Real-time PCR followed by screening with focused DNA array analysis revealed up-regulated expression of glucokinase (1.7-fold), lipoprotein lipase (3.8-fold) and interleukin-6 (3.7-fold) and down-regulated expression of vascular endothelial growth factor-A (0.2-fold), type I collagen (0.5-fold), and transforming growth factor-_ (0.4-fold) in ATGL(-/-)mSMC compared to ATGL(+/+)mSMC. Next, ectopic gene transfer of human ATGL was attempted using doxycycline (Dox)-regulatable myc-DDK-tagged adenovirus vector (AdvATGL). AdvATGL infection resulted in a reduction of TG accumulation with elevated mitogenic response and SMA expression, and decreased in senescent cell numbers in ATGL(-/-)mSMC. Moreover, deviated gene expression pattern in ATGL(-/-)mSMC was potentially corrected. Our data suggest that ATGL(-/-)mSMC have a distinct phenotype that may be related to vascular pathogenesis. Plasticity of SMC phenotypes correlated to lipid metabolism could be a therapeutic target.

 


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    Lipase from porcine pancreas CAS:9001-62-1 Brown to beige powder