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Kanamycin A Sulfate CAS:25389-94-0 White crystalline powder

Short Description:

Catalog Number: XD90363
CAS: 25389-94-0
Molecular Formula: C18H36N4O11 · H2O4S
Molecular Weight: 582.58
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Product Detail

Product Tags

Catalog Number XD90363
Product Name Kanamycin A Sulfate
CAS 25389-94-0
Molecular Formula C18H36N4O11 · H2O4S
Molecular Weight 582.58
Storage Details 15 to 30 °C
Harmonized Tariff Code 29419000

 

Product Specification

Grade USP
pH 6.5-8.5
Bacterial endotoxin <0.60EU/mg
Loss on Drying 4.0% max
Solubility Freely soluble in water, insoluble in acetone in ethyl acetate and in benzene
Assay >750ug/mg
Sulphated Ash 11 - 17.7%
Residue on Ignition <1.0%
Specific optical rotation +112 - +123
Residual Solvents Ethanol 2000ppm Max
Total Impurities 3.0% max
Appearance White crystalline powder
Manufacturing Date TBC
Kanamycin B 1.5% max
Any unknown impurity 0.45% max
Microbial Limit 25cfu/g Max

 

Antimicrobial proteins/peptides have attracted much attention because of their potential use in the industrial setting. In the present study, a thermostable antimicrobial protein (BSAMP) was purified from the culture supernatant of Bacillus subtilis FB123 by ammonium sulfate precipitation, gel chromatography on Sephacryl S-200 High Resolution, and ion exchange chromatography on DEAE Sepharose Fast Flow column. The molecular weight of the purified BSAMP was 54 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis both in the absence and presence of β-mercaptoethanol. Its isoelectric point was determined to be 5.24 by isoelectric focusing electrophoresis. Periodic acid-Schiff staining revealed BSAMP to be a glycoprotein. Maximum activity was obtained at pH 6.0, with over 79% maximum activity retained at pH 3.0-5.0 and pH 7.0-9.0, respectively. BSAMP was shown to be highly thermostable, as its activity did not change obviously after treatment at 100 °C. However, i t was partially sensitive to papain, trypsin, and alkali proteases. Finally, the bacterial protein exhibited broad-spectrum antimicrobial activity against several pathogenic organisms. These findings suggested that BSAMP should be further developed as a natural antibacterial agent for disease prevention in aquiculture and agriculture.


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    Kanamycin A Sulfate CAS:25389-94-0 White crystalline powder