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High reputation 2-Nitrophenyl-Beta-D-Glucopyranoside - MOPS sodium salt Cas:71119-22-7 99.0% White free flowing powder – XD BIOCHEM

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High reputation 2-Nitrophenyl-Beta-D-Glucopyranoside - MOPS sodium salt Cas:71119-22-7 99.0% White free flowing powder – XD BIOCHEM Detail:

Catalog Number XD90080
Product Name MOPS sodium salt
CAS 71119-22-7
Molecular Formula C7H14NNaO4S
Molecular Weight 231.245
Storage Details Ambient
Harmonized Tariff Code 29349990

Product Specification

Appearance White free flowing powder
Water content KF <1%
Assay (Titration, dry-basis >99.0%

New high-performance liquid chromatography assay for glycosyltransferases based on derivatization with anthranilic acid and fluorescence detection.

Assays were developed using the unique labeling chemistry of 2-aminobenzoic acid (2AA; anthranilic acid, AA) for measuring activities of both β1-4 galactosyltransferase (GalT-1) and α2-6 sialyltransferase (ST-6) by high-performance liquid chromatography (HPLC) with fluorescence detection (Anumula KR. 2006. Advances in fluorescence derivatization methods for high-performance liquid chromatographic analysis of glycoprotein carbohydrates. Anal Biochem. 350:1-23). N-Acetylglucosamine (GlcNAc) and N-acetyllactosamine were used as acceptors and uridine diphosphate (UDP)-galactose and cytidine monophosphate (CMP)-N-acetylneuraminic acid (NANA) as donors for GalT-1 and ST-6, respectively. Enzymatic products were labeled in situ with AA and were separated from the substrates on TSKgel Amide 80 column using normal-phase conditions. Enzyme units were determined from the peak areas by comparison with the concomitantly derivatized standards Gal-β1-4GlcNAc and NANA-α2-6 Gal-β1-4GlcNAc. Linearity (time and enzyme concentration), precision (intra- and interassay) and reproducibility for the assays were established. The assays were found to be useful in monitoring the enzyme activities during isolation and purification. The assays were highly sensitive and performed equal to or better than the traditional radioactive sugar-based measurements. The assay format can also be used for measuring the activity of other transferases, provided that the carbohydrate acceptors contain a reducing end for labeling. An assay for glycoprotein acceptors was developed using IgG. A short HPLC profiling method was developed for the separation of IgG glycans (biantennary G0, G1, G2, mono- and disialylated), which facilitated the determination of GalT-1 and ST-6 activities in a rapid manner. Furthermore, this profiling method should prove useful for monitoring the changes in IgG glycans in clinical settings.


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It can be our duty to satisfy your preferences and successfully serve you. Your pleasure is our best reward. We have been looking forward to the go to for joint expansion for High reputation 2-Nitrophenyl-Beta-D-Glucopyranoside - MOPS sodium salt Cas:71119-22-7 99.0% White free flowing powder – XD BIOCHEM , The product will supply to all over the world, such as: Poland, Malta, The Swiss, Our focus on product quality, innovation, technology and customer service has made us one of undisputed leaders worldwide in the field. Bearing the concept of "Quality First, Customer Paramount, Sincerity and Innovation" in our mind, We have achieved great progress in the past years. Clients are welcomed to buy our standard products, or send us requests. You will be impressed by our quality and price. Please contact us now!
  • Company director has very rich management experience and strict attitude, sales staff are warm and cheerful, technical staff are professional and responsible,so we have no worry about product,a nice manufacturer.
    5 Stars By Stephen from Somalia - 2018.02.08 16:45
    The supplier abide the theory of "quality the basic, trust the first and management the advanced" so that they can ensure a reliable product quality and stable customers.
    5 Stars By Clara from Jordan - 2018.09.21 11:44
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    High reputation 2-Nitrophenyl-Beta-D-Glucopyranoside - MOPS sodium salt Cas:71119-22-7 99.0% White free flowing powder – XD BIOCHEM