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Geneticin disulfate CAS:108321-42-2 White powder

Short Description:

Catalog Number: XD90373
CAS: 108321-42-2
Molecular Formula: C20H40N4O10 2H2SO4
Molecular Weight: 692.71
Availability: In Stock
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Prepack: 1g USD10
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Product Detail

Product Tags

Catalog Number XD90373
Product Name Geneticin disulfate
CAS 108321-42-2
Molecular Formula C20H40N4O10 2H2SO4
Molecular Weight 692.71
Storage Details 2 to 8 °C
Harmonized Tariff Code 29419000

 

Product Specification

Assay 99%
Appearance White powder
For research use only, not for human use Research use only, not for human use

 

We developed a novel small interfering RNA (siRNA) delivery system using a ternary complex with polyethyleneimine (PEI) and γ-polyglutamic acid (γ-PGA), which showed silencing effect and no cytotoxicity. The binary complexes of siRNA with PEI were approximately 73-102 nm in particle size and 45-52 mV in ζ-potential. The silencing effect of siRNA/PEI complexes increased with an increase of PEI, and siRNA/PEI complexes with a charge ratio greater than 16 showed significant luciferase knockdown in a mouse colon carcinoma cell line regularly expressing luciferase (Colon26/Luc cells). However, strong cytotoxicity and blood agglutination were observed in the siRNA/Lipofectamine complex and siRNA/PEI16 complex. Recharging cationic complexes with an anionic compound was reported to be a promising method for overcoming these toxicities. We therefore prepared ternary complexes of siRNA with PEI (charge ratio 16) by the addition of γ-PGA to reduce cytotoxicity and deliver siRNA. As expected, the cytotoxicity of the ternary complexes decreased with an increase of γ-PGA content, which decreased the ζ-potential of the complexes. A strong silencing effect comparable to siRNA/Lipofectamine complex was discovered in ternary complexes including γ-PGA with an anionic surface charge. The high incorporation of ternary complexes into Colon26/Luc cells was confirmed with fluorescence microcopy. Having achieved knockdown of an exogenously transfected gene, the ability of the complex to mediate knockdown of an endogenous housekeeping gene, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), was assessed in B16-F10 cells. The ternary complex (siRNA/PEI16/γ-PGA12 complex) exhibited a significant GAPDH knockdown effect. Thus, we developed a useful siRNA delivery system.


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    Geneticin disulfate CAS:108321-42-2 White powder