Factory wholesale 1,2,3,4-Di-O-Isopropylidene-Alpha-D-Galactopyranose – 4-Nitrophenyl-beta-D-glucopyranoside 99% CAS:2492-87-7 White Crystalline Powder – XD BIOCHEM
Factory wholesale 1,2,3,4-Di-O-Isopropylidene-Alpha-D-Galactopyranose – 4-Nitrophenyl-beta-D-glucopyranoside 99% CAS:2492-87-7 White Crystalline Powder – XD BIOCHEM Detail:
| Catalog Number | XD90022 |
| Product Name | 4-Nitrophenyl-beta-D-glucopyranoside |
| CAS | 2492-87-7 |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 |
| Storage Details | -15 to -20 °C |
| Harmonized Tariff Code | 29400000 |
Product Specification
| Melting Point | 160-170°C |
| Water Content | ≤2.0% |
| Solubility in water | Clear,colourless solution |
| Appearance | White crystaline powder |
| Assay | 99% |
A novel β-glucosidase gene, bgl1G5, was cloned from Phialophora sp. G5 and successfully expressed in Pichia pastoris. Sequence analysis indicated that the gene consists of a 1,431-bp open reading frame encoding a protein of 476 amino acids. The deduced amino acid sequence of bgl1G5 showed a high identity of 85 % with a characterized β-glucosidase from Humicola grisea of glycoside hydrolase family 1. Compared with other fungal counterparts, Bgl1G5 showed similar optimal activity at pH 6.0 and 50 °C and was stable at pH 5.0–9.0. Moreover, Bgl1G5 exhibited good thermostability at 50 °C (6 h half-life) and higher specific activity (54.9 U mg–1). The K m and V max values towards p-nitrophenyl β-D-glucopyranoside (pNPG) were 0.33 mM and 103.1 μmol min–1 mg–1, respectively. The substrate specificity assay showed that Bgl1G5 was highly active against pNPG, weak on p-nitrophenyl β-D-cellobioside (pNPC) and p-nitrophenyl-β-D-galactopyranoside (ONPG), and had no activity on cellobiose.
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By Michaelia from Estonia - 2018.06.12 16:22
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By April from Mombasa - 2017.11.12 12:31
