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Factory Price Patent Expired - L-Isoleucine Cas: 73-32-5 98.5-101.5% White powder – XD BIOCHEM

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Factory Price Patent Expired - L-Isoleucine Cas: 73-32-5 98.5-101.5% White powder – XD BIOCHEM Detail:

Catalog Number XD90303
Product Name L-Isoleucine

CAS

73-32-5

Molecular Formula

C6H13NO2

Molecular Weight

131.17292
Storage Details Ambient
Harmonized Tariff Code 29224985

 

Product Specification

Specific rotation +38.9 to +41.8
Heavy metals <15ppm
AS <1.5ppm
Ph 5.5 – 7
Loss on Drying <0.3%
Sulfate <0.03%
Assay 99%
Iron <30ppm
Residue on Ignition <0.3%
Cl <0.05%
Appearance White/ off white powder

 

Genome evolution in intracellular microbial symbionts is characterized by gene loss, generating some of the smallest and most gene-poor genomes known. As a result of gene loss these genomes commonly contain metabolic pathways that are fragmented relative to their free-living relatives. The evolutionary retention of fragmented metabolic pathways in the gene-poor genomes of endosymbionts suggests that they are functional. However, it is not always clear how they maintain functionality. To date, the fragmented metabolic pathways of endosymbionts have been shown to maintain functionality through complementation by host genes, complementation by genes of another endosymbiont and complementation by genes in host genomes that have been horizontally acquired from a microbial source that is not the endosymbiont. Here, we demonstrate a fourth mechanism.We investigate the evolutionary retention of a fragmented pathway for the essential nutrient pantothenate (vitamin B5) in the pea aphid, Acyrthos iphon pisum endosymbiosis with Buchnera aphidicola. Using quantitative analysis of gene expression we present evidence for complementation of the Buchnera pantothenate biosynthesis pathway by host genes. Further, using complementation assays in an Escherichia coli mutant we demonstrate functional replacement of a pantothenate biosynthesis enzyme, 2-dehydropantoate 2-reductase (E.C. 1.1.1.169), by an endosymbiont gene, ilvC, encoding a substrate ambiguous enzyme.Earlier studies have speculated that missing enzyme steps in fragmented endosymbiont metabolic pathways are completed by adaptable endosymbiont enzymes from other pathways. Here, we experimentally demonstrate completion of a fragmented endosymbiont vitamin biosynthesis pathway by recruitment of a substrate ambiguous enzyme from another pathway. In addition, this work extends host/symbiont metabolic collaboration in the aphid/Buchnera symbiosis from amino acid metabolism to include vitamin biosynthesis.


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Factory Price Patent Expired - L-Isoleucine  Cas: 73-32-5  98.5-101.5%  White powder – XD BIOCHEM detail pictures


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    Factory Price Patent Expired - L-Isoleucine Cas: 73-32-5 98.5-101.5% White powder – XD BIOCHEM