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Dithizone CAS:60-10-6 80% Black blue powder

Short Description:

Catalog Number: XD90464
CAS: 60-10-6
Molecular Formula: C13H12N4S
Molecular Weight: 256.33
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Product Detail

Product Tags

Catalog Number XD90464
Product Name Dithizone
CAS 60-10-6
Molecular Formula C13H12N4S
Molecular Weight 256.33
Storage Details Ambient
Harmonized Tariff Code 29309098

 

Product Specification

Loss on Drying ≤5%
Appearance Black blue powder
Assay 80%
Residue on Ignition ≤0.2%

 

Mesenchymal stem cells (MSCs) have been reported to be an attractive source for the generation of transplantable surrogate β cells. A murine embryonic mesenchymal progenitor cell line C3H10T1/2 has been recognized as a model for MSCs, because of its multi-lineage differentiation potential. The purpose of this study was to explore whether C3H/10T1/2 cells have the potential to differentiate into insulin-producing cells (IPCs). Here, we investigated and compared the in vitro differentiation of rat MSCs and C3H10T1/2 cells into IPCs. After the cells underwent IPC differentiation, the expression of differentiation markers were detected by immunocytochemistry, reverse transcription-polymerase chain reaction (RT-PCR), quantitative real-time RT-PCR (qRT-PCR) and Western blotting. The insulin secretion was evaluated by enzyme-linked immunosorbent assay (ELISA). Furthermore, these differentiated cells were transplanted into streptozotocin-induced diabetic mice and their biological functions wer e tested in vivo. This study reports a 2-stage method to generate IPCs from C3H10T1/2 cells. Under specific induction conditions for 7-8 days, C3H10T1/2 cells formed three-dimensional spheroid bodies (SBs) and secreted insulin, while generation of IPCs derived from rat MSCs required a long time (more than 2 weeks). Furthermore, these IPCs derived from C3H10T1/2 cells were injected into diabetic mice and improves basal glucose, body weight and exhibited normal glucose tolerance test. The present study provided a simple and faithful in vitro model for further investigating the mechanism underlying IPC differentiation of MSCs and cell replacement therapy for diabetes.


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    Dithizone CAS:60-10-6 80% Black blue powder