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Dimethyl yellow CAS:60-11-7 Yellow to Orange to brown powder

Short Description:

Catalog Number: XD90467
CAS: 60-11-7
Molecular Formula: C14H15N3
Molecular Weight: 225-29
Availability: In Stock
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Prepack: 25g USD10
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Product Detail

Product Tags

Catalog Number XD90467
Product Name Dimethyl yellow
CAS 60-11-7
Molecular Formula C14H15N3
Molecular Weight 225-29
Storage Details Ambient
Harmonized Tariff Code 29270000

 

Product Specification

Melting Point 105-116 Deg C
Appearance Yellow to Orange to brown powder
Assay 99%
Loss on Drying <1.0%
Strength 98.67%
Colour Change Red @ pH 2.9, Yellow @ pH 4.9
Sulfated ash 0.4%
Lamda Max 400nm (in methanol)

 

The membrane topology of the colicin E1 channel domain was studied by fluorescence resonance energy transfer (FRET). The FRET involved a genetically encoded fluorescent amino acid (coumarin) as the donor and a selectively labeled cysteine residue tethered with DABMI (4-(dimethylamino)phenylazophenyl-4'-maleimide) as the FRET acceptor. The fluorescent coumarin residue was incorporated into the protein via an orthogonal tRNA/aminoacyl-tRNA synthetase pair that allowed selective incorporation into any site within the colicin channel domain. Each variant harbored a stop (TAG) mutation for coumarin incorporation and a cysteine (TGT) mutation for DABMI attachment. Six interhelical distances within helices 1-6 were determined using FRET analysis for both the soluble and membrane-bound states. The FRET data showed large changes in the interhelical distances among helices 3-6 upon membrane association providing new insight into the membrane-bound structure of the channel domain. In general, the coumarin-DABMI FRET interhelical efficiencies decreased upon membrane binding, building upon the umbrella model for the colicin channel. A tentative model for the closed state of the channel domain was developed based on current and previously published FRET data. The model suggests circular arrangement of helices 1-7 in a clockwise direction from the extracellular side and membrane interfacial association of helices 1, 6, 7, and 10 around the central transmembrane hairpin formed by helices 8 and 9.


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    Dimethyl yellow CAS:60-11-7 Yellow to Orange to brown powder