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dCMP, 2′-Deoxycytidine 5′-monophosphate, free acid CAS:1032-65-1

Short Description:

Catalog Number: XD90549
CAS: 1032-65-1
Molecular Formula: C9H14N3O7P
Molecular Weight: 307.20
Availability: In Stock
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Prepack: 1g USD20
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Product Detail

Product Tags

Catalog Number XD90549
Product Name dCMP, 2'-Deoxycytidine 5'-monophosphate, free acid
CAS 1032-65-1
Molecular Formula C9H14N3O7P
Molecular Weight 307.20
Storage Details -15 to -20 °C
Harmonized Tariff Code 29349990

 

Product Specification

Appearance Solid
Assay 99%

 

Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine (PSI-6130) is a potent specific inhibitor of hepatitis C virus (HCV) RNA synthesis in Huh-7 replicon cells. To inhibit the HCV NS5B RNA polymerase, PSI-6130 must be phosphorylated to the 5'-triphosphate form. The phosphorylation of PSI-6130 and inhibition of HCV NS5B were investigated. The phosphorylation of PSI-6130 by recombinant human 2'-deoxycytidine kinase (dCK) and uridine-cytidine kinase 1 (UCK-1) was measured by using a coupled spectrophotometric reaction. PSI-6130 was shown to be a substrate for purified dCK, with a Km of 81 microM and a kcat of 0.007 s-1, but was not a substrate for UCK-1. PSI-6130 monophosphate (PSI-6130-MP) was efficiently phosphorylated to the diphosphate and subsequently to the triphosphate by recombinant human UMP-CMP kinase and nucleoside diphosphate kinase, respectively. The inhibition of wild-type and mutated (S282T) HCV NS5B RNA polymerases was studied. The steady-state inhibition constant (Ki) for PSI-61 30 triphosphate (PSI-6130-TP) with the wild-type enzyme was 4.3 microM. Similar results were obtained with 2'-C-methyladenosine triphosphate (Ki=1.5 microM) and 2'-C-methylcytidine triphosphate (Ki=1.6 microM). NS5B with the S282T mutation, which is known to confer resistance to 2'-C-methyladenosine, was inhibited by PSI-6130-TP as efficiently as the wild type. Incorporation of PSI-6130-MP into RNA catalyzed by purified NS5B RNA polymerase resulted in chain termination.


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    dCMP, 2′-Deoxycytidine 5′-monophosphate, free acid CAS:1032-65-1