Cytidine Cas:65-46-3 White crystalline powder 99%
Catalog Number | XD90586 |
Product Name | Cytidine |
CAS |
65-46-3 |
Molecular Formula |
C9H13N3O5 |
Molecular Weight |
243.22 |
Storage Details | Ambient |
Harmonized Tariff Code | 29349990 |
Product Specification
Appearance | White crystalline powder |
Assay | 99% |
Heavy metals | <20ppm |
Chemical Formula | C9H13N3O5 |
Loss on Drying | <0.5 % |
Residue on Ignition | <0.10 % |
Cellular supply of deoxynucleoside triphosphates (dNTPs) is crucial for DNA replication and repair. In this study, we investigated the role of CMP/UMP kinase (CMPK), an enzyme catalyzes CDP formation, in DNA repair. Knockdown of CMPK delays DNA repair during recovery from UV damage in serum-deprived cells but not in the cells without serum deprivation. Exogenous supply of cytidine or deoxycytidine facilitates DNA repair dependent on CMPK in serum-deprived cells, suggesting that the synthesis of dCDP or CDP determines the rate of repair. However, CMPK knockdown does not affect the steady state level of dCTP in serum-deprived cells. We then found the localization of CMPK at DNA damage sites and its complex formation with Tip60 and ribonucleotide reductase. Our analysis demonstrated that the N-terminal 32-amino-acid of CMPK is required for its recruitment to DNA damage sites in a Tip60-dependent manner. Re-expression of wild-type but not N-terminus deleted CMPK restores the efficiency of DNA repair in CMPK knockdown cells. We proposed that site-specific dCDP formation via CMPK provides a means to facilitate DNA repair in serum-deprived cells.