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China Supplier Amgen - L-Arginine CAS:74-79-3 99% White crystals or crystalline powder – XD BIOCHEM

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China Supplier Amgen - L-Arginine CAS:74-79-3 99% White crystals or crystalline powder – XD BIOCHEM Detail:

Catalog Number XD90323
Product Name L-Arginine
CAS 74-79-3
Molecular Formula C6H14N4O2
Molecular Weight 174.20
Storage Details Ambient
Harmonized Tariff Code 29252900

 

Product Specification

Appearance White crystals or crystalline powder
Assay 99%
Identification Infrared Absorption
Loss on Drying ≤ 0.5%
Chromatographic purity Not more than 0.5% of any individual impurity is found ; Not more than 2.0% of total impurities is found
Residue on Ignition ≤ 0.3%
Heavy metals ( PB ) ≤ 0.0015%
Iron (as Fe) ≤ 0.003%
Sulfate (as SO4) ≤ 0.03%
Usp grade USP 33
Chloride (CI) ≤ 0.05%
Specific rotation [ α ] D 2 5 +26.3 ° ~ +27.7 °

 

The mechanism of Mycobacterium smegmatis G (MbsG), a flavin-dependent l-lysine monooxygenase, was investigated under steady-state and rapid reaction conditions using primary and solvent kinetic isotope effects, substrate analogs, pH and solvent viscosity effects as mechanistic probes. The results suggest that l-lysine binds before NAD(P)H, which leads to a decrease in the rate constant for flavin reduction. l-lysine binding has no effect on the rate of flavin oxidation, which occurs in a one-step process without the observation of a C4a-hydroperoxyflavin intermediate. Similar effects were determined with several substrate analogs. Flavin oxidation is pH independent while the kcat/Km and kred/KD pH profiles for NAD(P)H exhibit single pKa values of ∼6.0, with increasing activity as the pH decreases. At lower pH, the enzyme becomes more uncoupled, producing more hydrogen peroxide and superoxide. Hydride transfer is partially rate-limiting at neutral pH and becomes more rate-limiting at lo w pH. An inverse solvent viscosity effect on kcat/Km for NAD(P)H was observed at neutral pH whereas a normal solvent viscosity effect was observed at lower pH. Together, the results indicate a unique mechanism where a rate-limiting and pH-sensitive conformational change occurs in the reductive half-reaction, which affects the efficiency of lysine hydroxylation.


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    5 Stars By Marjorie from Haiti - 2018.09.08 17:09
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    China Supplier Amgen - L-Arginine CAS:74-79-3 99% White crystals or crystalline powder – XD BIOCHEM