page_banner

Products

China Factory for Mes Sodium Salt - PUROMYCIN DIHYDROCHLORIDE Cas: 58-58-2 98% White powder – XD BIOCHEM

Short Description:


Product Detail

Product Tags

Related Video

Feedback (2)

We always work as a tangible team to ensure that we can provide you with the best quality and the best price for 68399-79-1, Gilead Sciences, Nitrotetrazolium Blue Chloride, We welcome new and old customers from all walks of life to contact us for future business relationships and mutual success!
China Factory for Mes Sodium Salt - PUROMYCIN DIHYDROCHLORIDE Cas: 58-58-2 98% White powder – XD BIOCHEM Detail:

Catalog Number XD90195
Product Name Puromycin dihydrochloride 

CAS

58-58-2

Molecular Formula

C22H31Cl2N7O5

Molecular Weight

544.431
Storage Details -15 to -20 °C
Harmonized Tariff Code 29419000

 

Differentiation of orbital fibroblasts into mature adipocytes and subsequent accumulation of adipose tissue has been shown in the progression of Graves’ orbitopathy (GO). Autophagy is involved in adipogenesis, but little is known about the role of autophagy in the initiation and progression of GO. The aim of this study is to investigate the role of autophagy in the pathogenesis of GO.Orbital adipose/connective tissue explants from patients with GO and from normal subjects, as well as isolated orbital fibroblasts, were analyzed. Adipogenesis was induced using differentiating medium with or without hydrogen peroxide, and autophagy was manipulated using bafilomycin A1 and Atg5-targeted short hairpin RNA (shRNA). Autophagosomes were identified by electron microscopy. Expression of autophagy-related genes and adipogenesis-related transcription factors were analyzed by real time reverse transcription-polymerase chain reaction and/or Western blot analysis. Lipid droplet accumulation was exami ned by Oil Red O staining.Autophagic vacuoles were more abundant in GO cells than in non-GO cells (p<0.05). Expression of autophagy-related genes was significantly higher in GO tissues and cells than in their non-GO counterparts, respectively. Interleukin-1β increased LC3-II, p62, and Atg7 protein in GO cells. Autophagosome accumulation was shown at day 4 of adipogenesis and decreased by day 10, along with lipid droplet formation. Expression of LC3 and p62 proteins increased within 48 hours of differentiation and diminished gradually from day 4 to 10. Bafilomycin A1 treatment and Atg5 knockdown by shRNA inhibited lipid droplet accumulation and suppressed expression of adipogenic markers.Autophagy was increased in GO tissue and cells compared to non-GO tissue and cells, suggesting that autophagy plays a role in GO pathogenesis. Autophagy manipulation may be a therapeutic target for GO.

4-Nitrophenyl-N-acetyl-β- D-glucosaminide is a useful substrate for rapid colorimetric assay of N-acetyl-b-glucosaminidase activity in human urine. Chromogenic β-Glucosaminidase substrate yielding a yellow solution upon cleavage, employed particularly in yeasts and molds.


Product detail pictures:

China Factory for Mes Sodium Salt - PUROMYCIN DIHYDROCHLORIDE  Cas: 58-58-2  98% White powder  – XD BIOCHEM detail pictures


Related Product Guide:

Our progress depends around the innovative machines, great talents and consistently strengthened technology forces for China Factory for Mes Sodium Salt - PUROMYCIN DIHYDROCHLORIDE Cas: 58-58-2 98% White powder – XD BIOCHEM , The product will supply to all over the world, such as: Vietnam, Armenia, Panama, To have much more enterprise. ompanions, we've got updated the item list and seek for optimistic co-operation. Our web-site shows the latest and complete information and facts about our goods list and company. For further acknowledge, our consultant service group in Bulgaria will reply to all of the inquiries and complications immediately. They're going to make their finest effort to meet buyers need. Also we support the delivery of absolutely free samples. Business visits to our business in Bulgaria and factory are generally welcome for a win-win negotiation. Hope to expertise a happy company co-operation perform with you.
  • High Quality, High Efficiency, Creative and Integrity, worth having long-term cooperation! Looking forward to the future cooperation!
    5 Stars By Esther from India - 2018.06.09 12:42
    We feel easy to cooperate with this company, the supplier is very responsible, thanks.There will be more in-depth cooperation.
    5 Stars By Lena from Estonia - 2018.02.04 14:13
    Close

    China Factory for Mes Sodium Salt - PUROMYCIN DIHYDROCHLORIDE Cas: 58-58-2 98% White powder – XD BIOCHEM