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China Cheap price Pnpg - 4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9 White Crystalline Powder 98% – XD BIOCHEM

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China Cheap price Pnpg - 4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9 White Crystalline Powder 98% – XD BIOCHEM Detail:

Catalog Number XD900012
Product Name 4-Nitrophenyl-beta-D-xylopyranoside
CAS 2001-96-9
Molecular Formula C11H13NO7
Molecular Weight 271.23
Storage Details -2 to -8 °C
Harmonized Tariff Code 29400000

Product Specification

Water 2% max
Specific rotation -54 to -58
Appearance White crystaline powder
Solubility, 1% Water Clear, colourless solution
Purity HPLC >98%

Biosynthesis of proteoglycans and glycosaminoglycans in the presence of p-nitrophenyl-xyloside was studied using a primary rat ovarian granulosa cell culture system. Addition of p-nitrophenyl-xyloside into cell culture medium caused about a 700% increase of [35S]sulfate incorporation (ED50 at 0.03 mM) into macromolecules, which included free chondroitin sulfate chains initiated on xyloside and native proteoglycans. Free chondroitin sulfate chains initiated on xyloside were almost exclusively secreted into the medium. The molecular size of chondroitin sulfate chains decreased from 40,000 to 21,000 as the total [35S]sulfate incorporation was enhanced, suggesting that enhanced synthesis of chondroitin sulfate perturbed the normal mechanism of glycosaminoglycan chain termination. Biosynthesis of heparan sulfate proteoglycans was reduced by approximately 50%, likely due to competition at the level of UDP-sugar precursors. [35S]Sulfate incorporation was shut down by the addition of cycloheximide with an initial half time of approximately 2 hr in the presence of xyloside, while that in the absence of xyloside was about 20 min. The difference likely reflects the turnover rate of glycosaminoglycan synthesizing capacity as a whole. The turnover rate of glycosaminoglycan synthesizing capacity observed in ovarian granulosa cells was much shorter than that observed in chondrocytes, reflecting the relative dominance of proteoglycan biosynthetic activity in the total metabolic activity of the cells.


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    China Cheap price Pnpg - 4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9 White Crystalline Powder 98% – XD BIOCHEM