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Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane Cas: 6976-37-0 99%

Short Description:

Catalog Number: XD90111
Cas: 6976-37-0
Molecular Formula: C8H20NO5
Molecular Weight: 210.2476
Availability: In Stock
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Prepack: 100g USD20
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Product Detail

Product Tags

Catalog Number XD90111

Product Name

BIS-TRIS (Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane)

CAS

6976-37-0

Molecular Formula

C8H20NO5

Molecular Weight

210.2476

Storage Details

Ambient

Harmonized Tariff Code

2922190090

 

Product Specification

Heavy metals <10ppm
Residue on Ignition <0.1%
Pka 6.3-6.7
Appearance White Crystalline Powder
Solubility (1M in H20) Clear and Complete
pH (1M in H2O) 9.0-11.0
Moisture (LOD) 1.0% max.
Assay (Titration on dry basis)

99.0% min.

 

Buffer, used for the detection of creatine-creatinase; also used for effective protection of hemoglobin in freeze-drying; catholyte, used in IEF/2-D gel electrophoresis.

 

Glial cell line-derived neurotrophic factor (GDNF) is a potent inhibitor of ethanol consumption and relapse, and GDNF heterozygous knockout mice display increased reward sensitivity to ethanol and consume more ethanol after a period of abstinence than their wild-type littermates. Here, we tested whether ethanol alters GDNF expression in the ventral tegmental area (VTA; GDNF's site of action) and/or the nucleus accumbens (NAc; the main source of GDNF), and if so, determine the role of the endogenous growth factor in the regulation of ethanol consumption. Systemic administration of ethanol increased GDNF expression and protein levels in the VTA, but not the NAc. Additionally, GDNF levels were elevated after an ethanol-drinking session in rats that consumed ethanol in the intermittent-access two-bottle choice procedure for 1 week, but not 7 weeks. Deprivation following 7 weeks of excessive ethanol intake reduced GDNF levels, while a short ethanol binge drinking period following deprivation upregulated GDNF expression. Importantly, knockdown of GDNF within the VTA using adenovirus expressing short hairpin RNA facilitated the escalation of ethanol drinking by ethanol-naïve rats, but not by rats with a history of excessive ethanol consumption. These results suggest that during initial ethanol-drinking experiences, GDNF in the VTA is increased and protects against the development of excessive ethanol intake. However, the growth factor's protective response to ethanol breaks down after protracted excessive ethanol intake and withdrawal, resulting in persistent, excessive ethanol consumption.


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    Bis[2-Hydroxyethyl] imino Tris-(Hydroxymethyl)-methane Cas: 6976-37-0 99%