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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt Cas:6578-06-9 99% White to light brown powder

Short Description:

Catalog Number: XD90137
Cas: 6578-06-9
Molecular Formula: C8H5BrClNO4P·C7H10N
Molecular Weight: 433.62
Availability: In Stock
Price:  
Prepack: 5g USD20
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Product Detail

Product Tags

Catalog Number XD90137
Product Name BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt
CAS 6578-06-9
Molecular Formula C8H5BrClNO4P·C7H10N
Molecular Weight 433.62
Storage Details -15 to -20 °C
Harmonized Tariff Code 29339980

 

Product Specification

Appearance White/light brown powder
Assay ≥ 99%
Melting point 194-195 ºC
Boiling point 580.2°C at 760 mmHg
Flash point 304.7°C
Solubility DMF: 20 mg/mL
Solubility soluble in dimethylformamide, insoluble in water.
Sensitivity Sensitive to light, sensitive to air, and sensitive to heat

 

Biological activity: BCIP (BCIP p-toluidine salt; X-phosphate p-toluidine salt) is a chromogenic substrate for colorimetric detection of alkaline phosphatase activity.

Shiga toxins (Stxs) are produced by enterohemorrhagic Escherichia coli (EHEC), which cause human infections with an often fatal outcome. Vero cell lines, derived from African green monkey kidney, represent the gold standard for determining the cytotoxic effects of Stxs. Despite their global use, knowledge about the exact structures of the Stx receptor glycosphingolipids (GSLs) and their assembly in lipid rafts is poor. Here we present a comprehensive structural analysis of Stx receptor GSLs and their distribution to detergent-resistant membranes (DRMs), which were prepared from Vero-B4 cells and used as lipid raft equivalents. We identified globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer) as the GSL receptors for Stx1a, Stx2a, and Stx2e subtypes using TLC overlay detection combined with MS. The uncommon Stx receptor, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), which was specifically recognized (in addition to Gb3Cer and Gb4Cer) by Stx2e, was fully structurally characterized. Lipoforms of Stx receptor GSLs were found to mainly harbor ceramide moieties composed of sphingosine (d18:1) and C24:0/C24:1 or C16:0 fatty acid. Moreover, co-occurrence with lipid raft markers, SM and cholesterol, in DRMs suggested GSL association with membrane microdomains. This study provides the basis for further exploring the functional impact of lipid raft-associated Stx receptors for toxin-mediated injury of Vero-B4 cells.

 


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    BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt Cas:6578-06-9 99% White to light brown powder