Acridine orange, hemi salt Cas: 10127-02-3 Short
Catalog Number | XD90520 |
Product Name | Acridine orange, hemi salt |
CAS |
10127-02-3 |
Molecular Formula |
C17H20ClN3·½ZnCl2 |
Molecular Weight |
369.96 |
Storage Details | Ambient |
Harmonized Tariff Code | 32129000 |
Product Specification
Appearance |
Deep yellow/red/ brown powder |
Assay |
99% |
Loss on Drying |
<10% |
Specific absorption |
Min 1200 |
Wavelength of maximum absorption |
488.0 - 498.0 |
Absorption Ration |
0.90 - 1.90 |
A system for screening cervical cytological preparations is described which employs the Leitz Texture Analyzer System (E. Leitz, Rockleigh, N. J.) quantitative staining with acridine orange, and a fluorescence standard. The instrumentation scans cells on microscope slides and detects objects which it interprets to be nuclei with excess total nuclear green fluorescence intensity (Previous results employing manual measurements have indicated that normal nuclei do not produce total nuclear green fluorescence greater than a specific absolute intensity level). Detected objects are identified by visual observation. Cells (102,000) from 65 patients (29 normal, 36 abnormal) have been examined. In each abnormal sample, at least one abnormal cell was detected. In over half of the samples, three or fewer other objects (e.g. clumps of polymorphonuclear leukocytes) were detected. These are easily distinguishable from single nuclei, and could be discarded by someone with minimal cytological training .