5-Sulfosalicylic acid dihydrate Cas: 5965-83-3 White to off-white crystal
Catalog Number | XD90230 |
Product Name | 5-Sulfosalicylic acid dihydrate |
CAS |
5965-83-3 |
Molecular Formula |
C7H10O8S |
Molecular Weight |
254.215 |
Storage Details | Ambient |
Harmonized Tariff Code |
29182900 |
Product Specification
Sulfate | 5.5% max |
Moisture | 22% max |
Appearance | White to off-white crystal |
Total acid | 99 - 104% |
Assay | 99% |
Polycations are explored as carriers to deliver therapeutic nucleic acids. Polycations are conventionally pharmacological inert with the sole function of delivering therapeutic cargo. This study reports synthesis of a self-immolative polycation (DSS-BEN) based on a polyamine analogue drug N(1),N(11)-bisethylnorspermine (BENSpm). The polycation was designed to function dually as a gene delivery carrier and a prodrug targeting dysregulated polyamine metabolism in cancer. Using a combination of NMR and HPLC, we confirm that the self-immolative polycation undergoes intracellular degradation into the parent drug BENSpm. The released BENSpm depletes cellular levels of spermidine and spermine and upregulates polyamine catabolic enzymes spermine/spermidine N(1)-acetyltransferase (SSAT) and spermine oxidase (SMO). The synthesized polycations form polyplexes with DNA and facilitate efficient transfection. Taking advantage of the ability of BENSpm to sensitize cancer cells to TNFα-induced apoptos is, we show that DSS-BEN enhances the cell killing activity of TNFα gene therapy. The reported findings validate DSS-BEN as a dual-function delivery system that can deliver a therapeutic gene and improve the outcome of gene therapy as a result of the intracellular degradation of DSS-BEN to BENSpm and the subsequent beneficial effect of BENSpm on dysregulated polyamine metabolism in cancer.