2022 High quality Onpg - 4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2 Light Yellow to White Powder 98% – XD BIOCHEM
2022 High quality Onpg - 4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2 Light Yellow to White Powder 98% – XD BIOCHEM Detail:
| Catalog Number | XD90021 |
| Product Name | 4-Nitrophenyl Beta-D-glucuronide |
| CAS | 10344-94-2 |
| Molecular Formula | C12H13NO9 |
| Molecular Weight | 315.23 |
| Storage Details | -15 to -20 °C |
| Harmonized Tariff Code | 29329900 |
Product Specification
| Purity(HPLC) | ≥98% |
| Appearance | Light yellow to white powder |
| HNMP | Conforms to structure |
| Kart Fischer | max 10% |
A beta-glucosidase gene (bgl3) from Streptomyces sp. QM-B814 (American Type Culture Collection 11238) has been cloned by functional complementation of a beta-glucosidase-negative mutant of Streptomyces lividans. An open-reading frame of 1440 nucleotides encoding a polypeptide of 479 amino acids was found by sequencing. The encoded protein (Bgl3) shows extensive similarity (over 45% identity) with beta-glycosidases from family-1 glycosyl hydrolases. The cloned enzyme, purified following ammonium sulphate precipitation and two chromatographic steps, is monomeric with molecular mass 52.6 kDa, as determined by mass spectrometry, and an isoelectric point of pI 4.4. The enzyme appears to be a beta-glucosidase with broad substrate specificity, is active on cellooligomers, and performs transglycosylation reactions. The estimated apparent Km values for p-nitrophenyl-beta-D-glucopyranoside and cellobiose are 0.27 mM and 7.9 mM, respectively. The Ki values for glucose and delta-gluconolactone, using p-nitrophenyl-beta-D-glucopyranoside as a substrate, are 65 mM and 0.08 mM, respectively. The purified enzyme has a pH optimum of pH 6.5 and the temperature optimum for activity is 50 degrees
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By Alexia from Madrid - 2017.09.16 13:44
