2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM
2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM Detail:
Catalog Number | XD900018 |
Product Name | 4-Nitrophenyl-alpha-D-galactopyranoside |
CAS | 7493-95-0 |
Molecular Formula | C12H15NO8 |
Molecular Weight | 301.25 |
Storage Details | -15 to -20 °C |
Harmonized Tariff Code | 29400000 |
Product Specification
Appearance | White to Orange to Green powder to crystal |
Assay | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for the detection of α-galactosidase activity. The amount of released pNP was significantly increased when 4-Nitrophenyl α-D-galactopyranoside was used as a substrate.
Using4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPfromthe4-Nitrophenylα-D-galactopyranosideissignificantlyhigherthanthatfromtheotherpNP-glycopyranosidesatpH4.0whereasnoactivitywasdetectedatpH7.Chemicalbook0.Thedigestionassaysusing4-nitrophenyl (pNP) glycopyranosidesasartificialsubstratesshowsthatyieldinliberatedgalactoseresiduesfrom4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, i.e.thepHlevelwhereyieldthresholdisdecreasedatmost.
Substrate of α-D-galactosidase (α-D-Galactosidase). Binds to the E. coli lactose protein carrier. Medium for α-D-galactose.
A rapid method for assessing the lytic activity of antimicrobial agents against yeast and fungi has been developed. The assay is based on the release of the intracellular enzyme, maltase (alpha-glucosidase). The released maltase activity was measured colorimetrically by the production of p-nitrophenol from p-nitrophenyl-alpha-D-glucopyranoside (PNPG). The lytic activity of different antimicrobial compounds was measured against yeast cells or germinating spores of filamentous fungi. Lytic anti-yeast activity could be detected within 20 min incubation at 30 degrees C against Saccharomyces cerevisiae, Candida albicans, and Cryptococcus neoformans. Lytic anti-fungal activity appeared after 2 h of incubation at 30 degrees C against germinating spores of Aspergillus niger and Botrytis cinerea. Whole cells of either yeast or fungi did not hydrolyze sufficient PNPG within 3 h at 30 degrees C to yield a detectable color change. Lytic activity of enzymes (e.g., Lyticase), antibiotics (e.g., Amphotericin B), and an antibiotic-producing strain of bacteria were detected using the assay. The anti-yeast assay has been adapted to a 96-well microtiter format. Both assays provided a rapid, sensitive, and reproducible detection of lytic anti-yeast and anti-fungal activity.
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We emphasize development and introduce new products into the market every year for 2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM , The product will supply to all over the world, such as: Florida, America, Kuwait, With more than 9 years of experience and a professional team, we have exported our products to many countries and regions all over the world. We welcome customers, business associations and friends from all parts of the world to contact us and seek cooperation for mutual benefits.
By Coral from Afghanistan - 2018.11.02 11:11
Good quality, reasonable prices, rich variety and perfect after-sales service, it's nice!
By Debby from Slovenia - 2017.09.16 13:44