page_banner

Products

2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM

Short Description:


Product Detail

Product Tags

Related Video

Feedback (2)

Our well-equipped facilities and great good quality regulate throughout all stages of manufacturing enables us to guarantee total buyer gratification for Micropacked Bed Hydrogenation, Disodium 4-[3-Methyl-N-(4-Sulfonatobutyl)Anilino]Butane-1-Sulfonate, Fca, We welcome customers all over the word to contact us for future business relationships. Our products are the best. Once Selected, Perfect Forever!
2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM Detail:

Catalog Number XD900018
Product Name 4-Nitrophenyl-alpha-D-galactopyranoside
CAS 7493-95-0
Molecular Formula C12H15NO8
Molecular Weight 301.25
Storage Details -15 to -20 °C
Harmonized Tariff Code 29400000

Product Specification

Appearance White to Orange to Green powder to crystal
Assay 99%

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside for the detection of α-galactosidase activity. The amount of released pNP was significantly increased when 4-Nitrophenyl α-D-galactopyranoside was used as a substrate.

Using4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPfromthe4-Nitrophenylα-D-galactopyranosideissignificantlyhigherthanthatfromtheotherpNP-glycopyranosidesatpH4.0whereasnoactivitywasdetectedatpH7.Chemicalbook0.Thedigestionassaysusing4-nitrophenyl (pNP) glycopyranosidesasartificialsubstratesshowsthatyieldinliberatedgalactoseresiduesfrom4-Nitrophenylα-D-galactopyranosidemainlyatpH4.0, i.e.thepHlevelwhereyieldthresholdisdecreasedatmost.

Substrate of α-D-galactosidase (α-D-Galactosidase). Binds to the E. coli lactose protein carrier. Medium for α-D-galactose.

A rapid method for assessing the lytic activity of antimicrobial agents against yeast and fungi has been developed. The assay is based on the release of the intracellular enzyme, maltase (alpha-glucosidase). The released maltase activity was measured colorimetrically by the production of p-nitrophenol from p-nitrophenyl-alpha-D-glucopyranoside (PNPG). The lytic activity of different antimicrobial compounds was measured against yeast cells or germinating spores of filamentous fungi. Lytic anti-yeast activity could be detected within 20 min incubation at 30 degrees C against Saccharomyces cerevisiae, Candida albicans, and Cryptococcus neoformans. Lytic anti-fungal activity appeared after 2 h of incubation at 30 degrees C against germinating spores of Aspergillus niger and Botrytis cinerea. Whole cells of either yeast or fungi did not hydrolyze sufficient PNPG within 3 h at 30 degrees C to yield a detectable color change. Lytic activity of enzymes (e.g., Lyticase), antibiotics (e.g., Amphotericin B), and an antibiotic-producing strain of bacteria were detected using the assay. The anti-yeast assay has been adapted to a 96-well microtiter format. Both assays provided a rapid, sensitive, and reproducible detection of lytic anti-yeast and anti-fungal activity.


Product detail pictures:

2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM detail pictures


Related Product Guide:

We emphasize development and introduce new products into the market every year for 2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM , The product will supply to all over the world, such as: Florida, America, Kuwait, With more than 9 years of experience and a professional team, we have exported our products to many countries and regions all over the world. We welcome customers, business associations and friends from all parts of the world to contact us and seek cooperation for mutual benefits.
  • With a positive attitude of "regard the market, regard the custom, regard the science", the company works actively to do research and development. Hope we have a future business relationships and achieving mutual success.
    5 Stars By Coral from Afghanistan - 2018.11.02 11:11
    Good quality, reasonable prices, rich variety and perfect after-sales service, it's nice!
    5 Stars By Debby from Slovenia - 2017.09.16 13:44
    Close

    2022 Good Quality X-Gal - 4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99% – XD BIOCHEM