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γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium salt Cas:63699-78-5 Light yellow microcrystalline powder

Short Description:

Catalog Number: XD90183
Cas: 63699-78-5
Molecular Formula: C12H12N3O7-.H4N+
Molecular Weight: 328.27
Availability: In Stock
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Prepack: 5g USD20
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Product Detail

Product Tags

Catalog Number XD90183
Product Name γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium salt
CAS 63699-78-5
Molecular Formula C12H12N3O7-.H4N+
Molecular Weight 328.27
Storage Details Ambient

 

Product Specification

Appearance Light yellow microcrystalline powder
Assay 99%
Melting point 187ºC ± 2.0ºC
Boiling point 718.3°C at 760 mmHg
Flash point 368.5 °C
Solubility H2O: 100 mg/mL, clear, yellow-green

 

The kinetics of human serum gamma-glutamyltransferase (EC 2.3.2.2) were investigated, with use of glycylglycine as a gamma-glutamyl acceptor substrate and gamma-glutamyl-4-nitroanilide and its carboxy derivative, gamma-glutamyl-3-carboxy-4-nitroanilide, as donor substrates. The simultaneous occurrence of both gamma-glutamyltransfer and autotransfer was established by descending paper chromatography. Constant-ratio double-reciprocal plots confirm that the enzyme mechanism is nonsequential (ping-pong bi-bi). Inhibition by either donor was not found, and inhibition by glycylglycine was only observed at concentrations above those of clinical interest. Kinetic constants obtained by nonlinear regression analysis of initial velocity data were used to determine reagent substrate concentrations for the assay of this enzyme. An assay with use of 4 mmol of gamma-glutamyl-3-carboxy-4-nitroanilide and 100 mmol of glycylglycine per liter yielded equivalent activities to those by assay with use of 4 mmol of gamma-glutamyl-4-nitroanilide and 40 mmol of glycylglycine per liter. These concentrations of the carboxy donor and glycylglycine are also "cost optimal" and present no procedural problems when used.


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    γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium salt Cas:63699-78-5 Light yellow microcrystalline powder